Plant Cryopreservation: A Practical Guide

Preface

Acknowledgements

Contents

Contributors

Section I: Introduction to Cryopreservation

Cryopreservation—Practical Considerations

Cryopreservation Theory

Development of PVS-Based Vitrification and Encapsulation– Vitrification Protocols

The Development of Encapsulation Dehydration

Controlled Rate Cooling

Section II. Cryopreservation Protocols for Working Laboratories

Cryopreservation of Algae

Cryopreservation of Bryophytes and Ferns

Cryopreservation of Dedifferentiated Cell Cultures

Cryopreservation of Embryogenic Cultures

Cryopreservation of Excised Embryos and Embryonic Axes

Cryopreservation of Monocots

Cryopreservation of Herbaceous Dicots

Cryopreservation of Temperate Berry Crops

Cryopreservation of In Vitro Tissues of Deciduous Forest Trees

Cryopreservation of Fruit and Ornamental Trees

Cryopreservation of Dormant Buds

Cryopreservation of Pollen

Cryopreservation of Recalcitrant ( i. e. Desiccation- Sensitive) Seeds

Cryopreservation of Orthodox (Desiccation Tolerant) Seeds

Index